AUTOMATED YEAST DISSECTION CHIP

FEATURES  

  • Capable of trapping up to 8,000 individual yeast cells
  • Tracks roughly 500 yeast cells’ life spans for 16 different strains in 3-4 days of culturing
  • Daughter cells are automatically separated to reduce labor and time cost


SPECIFICATIONS  

  • Material: Silicone-based organic polymer
  • Dimensions (L x W x H): 75 x 25 x 4 mm
  • Substrate: #1 Glass coverslip or glass slide
  • Number of single-cell traps: Total 8,320 (520 x 16 chambers)
  • Number of inlets: 4 media inlets, 16 individual inlets


OPERATION AND ANALYSIS  

  • Continuous imaging of budding yeasts throughout their entire lifespan
  • Compatible with high resolution fluorescent imaging microscopes for single-cell tracking
  • Consistent results with conventional microdissection approach


BENEFITS 

  • Time effective: analysis only takes 3-4 days 
  • Reduces labor: automated tracking of entire life span
  • High throughput: processes approximately 8,000 cells for 16 different strains
  • Imaging capability: high resolution fluorescent imaging

SMART ALIQUOTOR

FEATURES 

  • Convenient, rapid, selective, and damage-free isolation of single cells
  • Simplifies single-cell isolation used for genetic and clonal analysis
  • Compatible with cells that vary in size and have low or high density


SPECIFICATIONS

  • Material: Silicon-based organic polymer 
  • Dimensions (diameter): 60 mm 
  • Injection volume: <100 µl
  • Number of inlets: 1 media inlet


OPERATION AND ANALYSIS

  • Single-cell identification available 5 minutes after suspension injection
  • Cell retrieval ratio is close to 100%
  • A single target cell can be isolated from millions using tandem chips
  • Compatible with various types and sizes of cells 


BENEFITS

  • Standalone device for single-cell isolation
  • High yield, which reaches 30-40 cells
  • High cell viability
  • Identifiable single-cell isolation

CUSTOM DEVICE DESIGN & MANUFACTURING

FEATURES  

  • Develop and customize microfluidic devices for a specific application
  • Provide comprehensive device design and consulting services
  • Manufacturing available in biocompatible polymer, glass, and plastic


APPLICATION  

  • Live cell imaging chamber
  • Cell manipulation for trapping and pairing
  • Microwells and microchambers available for long term culturing
  • Cell isolation and separation micromixer for microreactions and protein extraction
  • Single-cell genomics and proteomics
  • Cargo delivery and gene manipulation
  • High-throughput drug screening

FACILITIES

  • Class 100 certified cleanroom
  • Device testing and processing room
  • Tissue culturing room

EQUIPMENT

  • Laser material processor
  • Complete set of instruments needed for soft lithography
  • Multichannel fluorescent microscope

 

OUR TECHNOLOGY

  • Materials available: Silicone-based organic polymer, Glass, and Thermoplastics
  • Device layout: Experienced microfluidic designer and optimized device work flow
  • Photolithography: Up to 5 µm structure resolution with 1:3 PDMS aspect ratio
  • Laser processing: Up to 50 µm resolution
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