HIGH-THROUGHPUT YEAST AGING ANALYSIS CHIP

DESCRIPTION:  

  • Traps up to 8,000 individual yeast cells
  • Track ~500 yeast cells’ whole life spans of 16 different strains in 3-4 days of culture
  • Automatic separation of daughter cells greatly reduces labor and time cost


FEATURES:  

  • 1 x 2 inches device bonded on glass slide
  • 4 modules
  • 16 chambers
  • 8,000 Yeast cell tracking
  • 96 hrs tracking time


OPERATION AND ANALYSIS:   

  • Continuous imaging of budding yeasts throughout their entire lifespan
  • Compatible with high resolution fluorescent imaging microscope for individual cells tracking
  • Consistent results with conventional microdissection approach


CAPABILITIES:   

  • Time effective: 3-4 days analysis time
  • Reducing labor: automated tracking of whole life span
  • High throughput: ~8,000 cells of 16 different stains
  • Imaging capability: high resolution fluorescent imaging

SMART ALIQUOTOR

DESCRIPTION: 

  • Convenient, rapid, selective, and damage-free isolation of single cells
  • Can culture isolated cell in the chip for phenotypic and genetic analysis
  • Is compatible with extremely low and high density, size, type of cells


SINGLE CELL SMART ALIQUOTOR

  • φ 60 mm flexible device on culture dish
  • 1 inlet 
  • 100 outlets
  • <100 µl injection
  • <1 µl retrieve volume


OPERATION 

  • <5 min from suspension injection to single cell identification
  • Close to 100 % cell retrieve ratio
  • Single target cell can be isolated from millions by using tandem chips
  • Applicable for various type and size of cells from primary, stem, bacteria, fungi and yeast, and plant


CAPABILITIES

  • Standalone device for single cell isolation
  • High yield and high throughput
  • High cell viability
  • Identifiable single cell isolation

CUSTOM DEVICE DESIGN AND MANUFACTURE

DESCRIPTION:  

  • Develop and customize microfluidic device for your specific application
  • Provide comprehensive device design and consulting service
  • Manufacture in biocompatible polymer, glass, and plastic


APPLICATION:  

  • Live cell imaging chamber
  • Cell manipulation for trapping and pairing
  • Micro well and micro chamber for long term culture
  • Cell isolation and separation Micromixer for micro reaction and protein extraction
  • Single cell genomics and proteomics
  • Cargo delivery and gene manipulation
  • High throughput drug screening

FACILITIES:  

  • Class 100 certified cleanroom
  • Device test room
  • Device process room
  • Tissue culture room

EQUIPMENTS:

  • Laser material processor
  • Complete instruments for soft lithography
  • Multichannel fluorescent microscop

 

OUR TECHNOLOGIES:

  • Material selection: PDMS, Glass, and Thermoplastics
  • Device layout: Experienced microfluidic designer Optimized device work flow
  • Photolithography: Up to 5 µm structure resolution 1:3 PDMS aspect ratio
  • Laser process: Up to 50 µm resolution
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