1CellAssay Lysis Kit

Ultra-Low Volume Single-Cell Isolation & Lysis Kit

SKU: K1-SCLS-KIT Categories: Single-Cell Cloning and Analysis, Single-Cell Kits, Single-Cell PCR & Sequencing Tag: Related-One

APPLICATIONS

Single-Cell Isolation from Cell Lines and Primary Cells
Single-Cell Lysis in a Small Volume
Single-Cell PCR & Sequencing
Single-Cell Multi-omics Assays

HIGHLIGHTS

Guarantee to Isolate 24 Single Cells
Isolate Single Cells in 2 μL Volume
Lyse Single Cells in 4 μL Volume

Order from Fisher

$200.00

Z

In Stock – Usually shipped next day

Available through global distributors

Features
Specifications
Documentations

Features

Easy operation by regular pipette in a sterile hood
Gentle flow keeps high single-cell viability & integrity
Compatible with cell size ≤ 50 μm and numbers ≤ 100
Isolate and lyse 24 single cells in less than 30 minutes
No special equipment or operation skills are required
No liquid backflow or cross-talk between wells

OPERATION AND ANALYSIS

Uniformly distribute bulk-cell suspension into 64 outlet wells
A single target cell can be isolated from millions using tandem operation
Cell retrieval ratio is close to 100%
Designed to isolate 24 single cells or more
Isolate single cells in 2 µL volume
Lyse single cells in 4 µL volume

Specifications

1CellDishTM-60mm (2 devices)

Material: Biocompatible polystyrene and PDMS
Sterilization: Sterile
Bottom Coating of Well: Tissue culture treated
# of Single Cells Can Be Isolated: ~ 20 single cells per device

Cell Lysis Buffer (200 µL)

10mM Tris pH 7.5, 130mM NaCl, 1% Trito n X-100™, 10mM NaF, 10mM NaPi, 10mM NaPPi
Sterile, filtered

8-Strip PCR Tubes (3 strips)

DNA, RNase & DNase free
compatible with 0.2 mL thermal cycler for qPCR/Real-Time PCR

Documentations

1CellAssay Lysis Kit Flyer
1CellAssay Lysis Kit Protocol