Cutting-Edge
MICROFLUIDIC BioChips
iBiochips develops microplates to integrate ultra-low volume assays and microfluidics engineering that achieve high efficiency and cutting-edge precision. We resolve traditional biotech challenges with innovative biochip approaches.
Products at Glance
1CellDish-60mm
Applications
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Single cell isolation
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Single cell PCR & Sequencing
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CRISPR single-cell cloning
Features
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Convenient, rapid, selective, and damage-free isolation of single cells
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Simplifies single-cell isolation used for genetic and clonal analysis
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Compatible with any cell size (≤ 50 µm) and have low or high density
PicoWell Dish-72K Wells
Applications
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Spheroid culture
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High-throughput cell imaging
- Single cell culture
Features
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Culture a large number of spheroids on a single dish, ideal for high-throughput experiments
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Promote cell aggregation into spheroids that mimic tissue structures
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Prevent cells and spheroids drifting and movement out of the field of view during live cell imaging experiments
1CellPlate-Glass Bottom
Applications
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Single cell isolation
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Single-cell imaging with confocal microscopy
Features
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#1.5 Glass bottom plate for high-resolution imaging
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Simplifies single-cell isolation used for genetic and clonal analysis
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Compatible with any cell size (≤ 50 µm) and have low or high density
Automated Dissection Screening Chip
Applications
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High-throughput replicative lifespan assay
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Genetic screening for longevity
Features
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Tracks the whole lifespan of over 10,000 yeast cells
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Daughters are automatically separated from their mothers
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Each channel contains 20 variations of trap dimensions for screening
1CellAssay-Chamber Slide
Applications
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Single-cell isolation and assay
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Single-cell culture and assay
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Fluorescent and microscopy analysis of living or fixed cells
Features
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Easy single-cell identification in 1.8 mm well
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Gentle microflow keeps high single-cell viability
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No cell transfer is needed prior to visualization/staining
Standard Hole Array
Applications
- Multiplex assay
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High-density microwell
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High-throughput cell culture and imaging
Features
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Easily assemble with many commonly used platforms, including microplates, Petri Dishes, microscope slides, chamber slides, coverslips, silicon wafers, and others with smooth surfaces.
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Can be reused by simple water washing and ethanol sterilization.
1CellPlate-96well
Applications
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Single cell isolation
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Cell line development
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Single cell multiomics
Features
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Ultra-low single-cell isolation volume: 2.5 µL/cell
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Large single-cell culture well: 6.5 mm diameter
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Compatible with any cell size (≤ 80 µm) and have low or high density
1CellAssay 3D Culture Kit
Applications
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Isolate single cells in hydrogel
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Single-cell 3D clonal culture
Features
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Innovative well-in-well design to ensure stable hydrogel-liquid exchange
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Gentle flow keeps high single-cell viability & integrity
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Compatible with cell size ≤ 80 μm and numbers ≤ 100
Single-Cell PCR & Sequencing
Successful single-cell isolation is a primary step for downstream single-cell genetic analysis, especially in the single-cell PCR which highly requires a convenient and reliable method to rapidly isolate a viable and intact cell into a specific PCR tube. Our 1CellDishTM and 1CellPlate® series of single-cell isolation products overcome current single-cell isolation technical challenges in operational complexity, time consumption, the requirement of special skill or an expensive instrument, damage of cell viability or integrity, and incompetence in the isolation of single cells into ultra-low volume liquids. The 1CellDishTM and 1CellPlate® series of products combine standard device format with innovative microfluidic design to provide multiple easy, rapid, and cost-effective methods to isolate single cells into ultra-low volumes for desired single-cell-based genetic analyses, including lysis, PCR, and sequencing. Million Microwell Device containing 1,000,000 ultra-small wells provides an ultra-high-throughput but low-cost method for single-cell RNA sequencing.
Single-Cell Culture
Single-cell cloning has a wide range of applications, such as the production of recombinant proteins, gene function studies, and drug screening. Single-cell isolation is a primary step for successful single-cell cloning from cell lines and primary cells. The 1CellPlate®-96well Ultra-Low Volume 96-Well Microfluidic Microplate has a standard 96-well plate format and a unique microfluidic well-in-well design that provides unprecedented simplicity and convenience in the single-cell isolation for stable cell line development, including cancer cells, hybridoma cells, CHO cells, CRISPR-edited cells, and stem cells.
3D cell culture closely resembles in vivo cell environments to provide more physiologically relevant information and more predictive data and therefore has gained increasing interest in drug toxicity testing and screening. 1CellAssay® Ultra-Low Volume Single-Cell Isolation & 3D Culture Kit, containing 5 devices of 1CellPlate®-96well, 5 Inlet Adaptor, and 2 mL of Hydrogel Matrix, provides a complete solution from easy single-cell isolation to efficient 3D cloning in a 2.8 µL of xeno-free hydrogel for generating spheroids and organoids.
Single-Cell Assays
Systematic investigation of cytokine signaling activity at the single-cell level is critical for intercellular communication in human health and disease. 1CellPlate®-Assay Ultra-Low Volume 96-Well Microfluidic Microplate combines ultra-small wells with microfluidic networks to meet the need for single-cell cytokine assays. The 1CellPlate®-Assay has many merits, such as easy single-cell isolation by patented microfluidic aliquoting, convenient single-cell identification in a single field-of-view microscope image, easy well sealing from two noticed microplate sides for excellent evaporation prevention, and single-cell incubation and cytokine detection in ultra-low volumes. The single-cell cytokine secretion can be collected and deeply analyzed by mass spectrometry. Moreover, the single cells with remarkable cytokine production can also be easily retrieved by regular pipette for downstream analysis, including cloning or PCR & Sequencing.
Automated Lifespan Assay
Advancing our understanding of the underlying molecular mechanisms of aging, as well as their contributions to age-associated diseases, will have a profound impact on public health. Studying the replicative aging phenomenon in the budding yeast Saccharomyces cerevisiae has led to significant findings on how aging is regulated by evolutionarily conserved enzymes and molecular pathways. However, the laborious and low-throughput methods of current yeast replicative lifespan assays limit their usefulness as a broad genetic screening platform for research on aging.
Automated Dissection Chip (AD-Chip) provides automated whole-lifespan tracking of over 10,000 single yeast cells for 20 different strains in 3 days with time-lapse microscopy. The AD-Chip enables high-resolution fluorescent imaging of single-cells during the entire aging process, allowing for investigation of gradual changes in molecular markers on the single-cell scale and high-throughput examination of the aging phenotype, including organelle morphology, gene expression, and protein localization to identify genetic or environmental factors that regulate lifespan.
Bacterial Endotoxin Testing Platform
Endotoxin, originating from the cell membrane of gram-negative bacteria, causes strong immune response resulting symptoms such as fever, nausea and septic shock. It is required by a regulation to test the level of endotoxin in medical products, including implants, human and animal parenteral drugs and vaccine, medical equipment, and all products labeled as non-pyrogenic.
iBiochips endotoxin testing platform offers more precise kinetic endotoxin assay with reduced reagent consumption. Innovative microfluidic flow control integrated in standard well plate eliminate source of experimental and environmental errors, while it is compatible with existing endotoxin test system.
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